Part:BBa_K2578710:Design

Protein coding sequence for Mouse metallothionein 1 (Mt1)

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal XbaI site found at 438
  Illegal PstI site found at 703
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  Illegal PstI site found at 703
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 282
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal XbaI site found at 438
  Illegal PstI site found at 703
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal XbaI site found at 438
  Illegal PstI site found at 703
  Illegal AgeI site found at 316
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

The sequence cannot be divided into 3

Source

NCBI

References

[1] https://www.ncbi.nlm.nih.gov/gene/17748

[2] https://parts.igem.org/Part:BBa_J23100

[3] https://parts.igem.org/Part:BBa_B0032

[4] https://parts.igem.org/Part:BBa_B0015

[5] N Thirumoorthy, KT Manisenthil Kumar, A Shyam Sundar, L Panayappan, Malay Chatterjee (2007). Metallothionein: An overview. World journal of gastroenterology, ISSN 1007-9327

[6] Almaguer-Cantú V1, Morales-Ramos LH, Balderas-Rentería I. (2011) Biosorption of lead (II) and cadmium (II) using Escherichia coli genetically engineered with mice metallothionein I. Water Sci Technol. 2011;63(8):1607-13.

[7] Dziegiel, P., Pula, B., Kobierzycki, C., Stasiolek, M., Podhorska-Okolow, M. (2016), Metallothioneins in Normal and Cancer Cells, Springer International Publishing, DOI: 10.1007/978-3-319-27472-0

[8] Almaguer-Cantú, V., Morales-Ramos, L. H., & Balderas-Rentería, I. (2011). Biosorption of lead (II) and cadmium (II) using Escherichia coli genetically engineered with mice metallothionein I. Water Science and Technology, 63(8), 1607-1613. doi:10.2166/wst.2011.225

[9] Jiang, Y., & Kang, Y. J. (2004). Metallothionein Gene Therapy for Chemical-Induced Liver Fibrosis in Mice. Molecular Therapy, 10(6), 1130-1139. doi:10.1016/j.ymthe.2004.08.011